Quantification of Saccharomyces cerevisiae viability using BacLight
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1Environmental Biotechnology Laboratory, Department of Civil Engineering The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China.
Abstract:
Yeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from the actual data, and those determined by flow-cytometry by 1.4%.
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