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Updated: Aug 23, 2026

Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
Published on: April 22, 2015
Milestones of neuronal development in the adult hippocampus
Gerd Kempermann1, Sebastian Jessberger, Barbara Steiner
1VolkswagenStiftung Research Group, Department of Experimental Neurology, Charité University Medicine Berlin, Schumannstrasse 20/21, 10117 Berlin, Germany. gerd.kempermann@mdc-berlin.de
Abstract:
Adult hippocampal neurogenesis originates from precursor cells in the adult dentate gyrus and results in new granule cell neurons. We propose a model of the development that takes place between these two fixed points and identify several developmental milestones. From a presumably bipotent radial-glia-like stem cell (type-1 cell) with astrocytic properties, development progresses over at least two stages of amplifying lineage-determined progenitor cells (type-2 and type-3 cells) to early postmitotic and to mature neurons. The selection process, during which new neurons are recruited into function, and other regulatory influences differentially affect the different stages of development.
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