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[Studies on random amplified polymorphic DNA fingerprinting of Cortex Magnoliae Officinalis]
Wensheng Liu1, Jianming Zhu, Bin He
1Guangdong Provincial People's Hospital, Guangzhou 510080.
Objective:
To identify Chinese traditional medicine (CTM) "Hou-pu" (Cortex Magnoliae Officinalis), its counterfeits and substitues.
Methods:
Total genomic DNA samples of ten plant species were amplified by randomly amplified polymorphic DNA (RAPD).
Results:
Ten samples were able to be distinguished through their amplified DNA banding patterns on the agarose gels after electrophoresis.
Conclusion:
RAPD is able to identify "Hou-pu", its counterfeits and substitutes quickly and truly, which is also quite valuable for correctly introducing plant.
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