[Supercritical CO2 extraction of fatty oils from bee pollen and its GC-MS analysis]

The extraction of fatty oils from bee pollen with supercritical CO2 was studied. The effects of extraction pressure, extraction temperature and the grinding size of the bee pollen on the yields were discussed. The optimal condition of this method was: extraction pressure 30MPa,extraction temperature 55 degrees C, separator I pressure 14MPa, separator I temperature 45 degrees C, separator II pressure 6MPa, separator II temperature 40 degrees C, extraction period 2 hours. Compared with the traditional solvent extraction, this method had some virtues, such as shortening the extraction period greatly, escaping oxidation of unsaturated fatty acid, and the products from which having better quality. With a GC-MS analysis, the main composition of the fatty oil from the bee pollen was: oleic acid, palmitic acid, linoic acid, pentacosane, octacosane and so on. The contents of alpha-linolenic acid in the fatty oils from separator I and separator II were 42% and 8.8%, which differed greatly. It proved that this method succeeded in extraction, separating and enriching alpha-linolenic acid.