Immobilized jerusalem artichoke (Helianthus tuberosus) tissue electrode for phenol detection

A tissue based biosensor for the determination of phenol was developed by using Jerusalem artichoke (Helianthus tuberosus) in combination with a dissolved oxygen (DO) probe. The tissue electrode response depends linearly on phenol concentration between 0.002 and 0.0101 microM in 10 min response time. Maximum electrode response was found in phosphate buffer at pH 8.0 and 35 degrees C. The reproducibility of the enzyme electrode was also tested by using standard phenol solutions (0.005 microM). The standard deviation (SD) and variation coefficient (cv) were calculated as +/- 1.4 x 10(-4) microM and 3.1%, respectively.