Related Experiment Video
Updated: Aug 23, 2026
Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
Published on: July 30, 2016
Effect of hypoxia on urocortin production in human gestational trophoblasts in vitro
Mei Y Choy1, Tse N Leung, Po S Leung
1Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong, SAR, China. meiychoy@cuhk.edu.hk
Problem:
Urocortin is produced by the placenta throughout pregnancy but its regulation remains unknown. The effect of hypoxia on placental urocortin production is not known. The aim of this study was to determine the effect of in vitro hypoxia on human trophoblastic urocortin production.
Method Of Study:
Placental explants and primary cultures were incubated in anaerobe hypoxic bags for 24 h in a humidified incubator. Urocortin peptide secretion and mRNA (messenger RNA) production was determined by enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction, respectively. Morphological and functional integrity was verified by immunohistochemical analysis of urocortin expression. Vascular endothelial growth factor expression was used to verify the generation of cellular hypoxia in our in vitro system.
Results:
Hypoxia did not affect urocortin secretion or mRNA expression in explant and single-cell cultures. Production was greater from first trimester than term explants and from single-cell primary cultures more than from explant cultures.
Conclusions:
Hypoxia does not influence human placental urocortin secretion or mRNA expression in vitro.
More Related Videos
11:44A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta
Published on: September 27, 2017
05:30Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis
Published on: November 8, 2024
