Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Automated orthogonal control system for electrospray ionization.

Gary A Valaskovic1, James P Murphy, Mike S Lee

  • 1New Objective, Inc., Woburn, Massachusetts 01801, USA. garyv@newobjective.com

Journal of the American Society for Mass Spectrometry
|July 28, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Science of Gun Policy: A Critical Synthesis of Research Evidence on the Effects of Gun Policies in the United States, Fifth Edition.

Rand health quarterly·2026
Same author

Caregiver Social Networks and Discontinued Support During COVID-19: A Multilevel Dyadic Analysis.

The journals of gerontology. Series B, Psychological sciences and social sciences·2026
Same author

Kinship, gender, and the social networks of caregivers for older adults.

The Gerontologist·2026
Same author

The Science of Gun Policy: A Critical Synthesis of Research Evidence on the Effects of Gun Policies in the United States, Fourth Edition.

Rand health quarterly·2024
Same author

Relationships of State Alcohol Policy Environments With Homicides and Suicides.

American journal of preventive medicine·2024
Same author

Issue alignment and partisanship in the American public: Revisiting the 'partisans without constraint' thesis.

Social science research·2021

This study introduces an automated electrospray ionization (ESI) system that maintains stable spray modes during chromatography. This innovation ensures consistent performance by real-time compensation for changing mobile phase conditions.

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Chromatography

Background:

  • Low-flow electrospray ionization (ESI) typically relies on electrostatic forces without sheath gas.
  • ESI exhibits complex spray morphologies due to various emission modes, with the Taylor cone-jet mode being optimal under stable conditions.
  • Gradient elution chromatography destabilizes the Taylor cone-jet mode due to changing mobile phase properties.

Purpose of the Study:

  • To characterize common spray modes in low-flow ESI for aqueous/organic mobile phases.
  • To develop an automated feedback-controlled ESI source for maintaining stable spray modes.
  • To enable real-time compensation for variations in mobile phase composition and flow rate.

Main Methods:

  • Orthogonal imaging techniques (strobed transmitted light and continuous scattered light) were used to characterize spray modes.

Related Experiment Videos

  • Image analysis correlated complementary illumination methods for qualitative and quantitative spray mode identification.
  • An image-processing algorithm controlled electrospray potential via a feedback loop for automated spray mode control.

    • Main Results:

    • The study identified and characterized common spray modes under varying conditions.
    • An automated feedback-controlled ESI source was successfully developed.
    • The system demonstrated self-starting and self-tuning capabilities, maintaining cone-jet or pulsed cone-jet modes.
    • Real-time compensation for mobile phase changes was achieved, ensuring stable electrospray.

    Conclusions:

    • Complementary imaging and image analysis provide a robust method for spray mode identification.
    • Automated feedback control is effective in maintaining stable electrospray modes during gradient elution chromatography.
    • The developed system enhances the robustness and reliability of low-flow ESI coupled with chromatography.