Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

BRAF mutations in conjunctival melanoma.

Heather Gear1, Hawys Williams, Ewan G Kemp

  • 1Tennent Institute of Ophthalmology, Gartnavel General Hospital, Glasgow, Scotland, United Kingdom.

Investigative Ophthalmology & Visual Science
|July 28, 2004
PubMed
Summary

The BRAF T1799A mutation, common in skin melanoma, was found in some conjunctival melanoma samples. This suggests conjunctival melanoma may share biological similarities with cutaneous melanoma.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of a national mesothelioma network on outcomes including survival.

Thorax·2026
Same author

Secondary extraocular extension of choroidal melanoma following primary photodynamic therapy.

Eye (London, England)·2026
Same author

Modeling Genetic Risk of β-Cell Dysfunction in Human Induced Pluripotent Stem Cells From Patients Carrying the MTNR1B Risk Variant.

Journal of pineal research·2025
Same author

In Response to: Reflections on ECPR in New Zealand: Past, Present and Future.

Emergency medicine Australasia : EMA·2025
Same author

Deletion of Hsd11b1 suppresses caloric restriction-induced bone marrow adiposity in male but not female mice.

The Journal of endocrinology·2024
Same author

Ocular Lichen Planus: A clinicopathologic review.

European journal of ophthalmology·2024

Area of Science:

  • Ophthalmology
  • Oncology
  • Genetics

Background:

  • Activating BRAF mutations are prevalent in cutaneous melanoma but absent in uveal melanoma.
  • Conjunctival melanoma shares clinical similarities with cutaneous melanoma.

Purpose of the Study:

  • To investigate the presence of the BRAF T1799A mutation in conjunctival melanoma.
  • To determine if conjunctival melanoma shares genetic features with cutaneous melanoma.

Main Methods:

  • DNA extraction from conjunctival melanoma tissue samples.
  • Seminested PCR amplification of BRAF exon 15.
  • Direct sequencing and comparison with wild-type BRAF sequences.

Main Results:

  • The BRAF T1799A (V600E) mutation was detected in 5 of 22 conjunctival melanoma patients.

Related Experiment Videos

  • No significant associations between BRAF mutation and clinicopathological features were found in this series.
  • Tumors with the mutation showed a trend towards larger diameter, greater invasion depth, and epithelioid cells.

    • Conclusions:

    • The BRAF T1799A mutation is present in a subset of conjunctival melanomas.
    • This finding suggests a biological link between conjunctival and cutaneous melanoma.
    • Conjunctival melanoma may share underlying genetic mechanisms with cutaneous melanoma.