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Updated: Jul 27, 2026
11:22
Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
Published on: August 23, 2013
Fibronectin binding and neutrophil aggregation in burn injury
P J Spagnuolo1, B Donofrio, W E Litzenberg
1Department of Medicine, Case Western Reserve University School of Medicine, MetroHealth Medical Center/Cleveland Metropolitan General Hospital, Ohio 44109.
The Journal of Surgical Research
|September 1, 1992
Summary
Activated neutrophils (PMN) bind fibronectin (Fn) to aggregate. Burn injury impairs this Fn binding and PMN aggregation, suggesting a role in thermal injury responses.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Fibronectin (Fn) is crucial for cell adhesion, particularly for neutrophils (PMN).
- Altered PMN adhesive function is observed in acute burn injury.
- The role of Fn in PMN aggregation following thermal injury requires investigation.
Purpose of the Study:
- To test if activated PMN develop binding sites for fibronectin, facilitating PMN aggregation.
- To investigate the role of fibronectin in the aggregation of PMN from burn patients.
Main Methods:
- Utilized radioiodinated plasma fibronectin to quantify Fn binding to PMN.
- Stimulated PMN with the chemotactic peptide n-formylmethionyl leucyl phenylalanine.
- Compared Fn binding and aggregation kinetics in healthy subjects and burn patients.
Main Results:
- Chemotactic peptide induced rapid, partially reversible, and nonsaturable fibronectin binding to PMN.
- Fibronectin binding and PMN aggregation exhibited similar kinetics and requirements.
- PMN from burn patients showed reduced aggregation and decreased fibronectin binding.
Conclusions:
- Activated PMN possess binding sites for fibronectin, mediating PMN aggregation.
- Impaired fibronectin binding to PMN may contribute to reduced aggregation in early-stage thermal injury.
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