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Related Experiment Videos

Glass technology for patch clamp electrodes.

J L Rae, R A Levis

    Methods in Enzymology
    |January 1, 1992
    PubMed
    Summary
    This summary is machine-generated.

    No single glass suits all cell recording needs. Borosilicate glasses are versatile, while specific types like Corning 8161 or high lead glasses excel in specialized applications like perforated-patch recordings.

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    Area of Science:

    • Materials Science
    • Neuroscience
    • Biophysics

    Background:

    • Glass properties significantly impact electrophysiological recordings.
    • Selecting appropriate glass is crucial for successful single-channel and whole-cell patch-clamp experiments.

    Purpose of the Study:

    • To evaluate various glass types for their suitability in electrophysiological cell recordings.
    • To provide guidance on selecting the optimal glass for different experimental conditions.

    Main Methods:

    • Review and comparison of electrical and thermal properties of different glass formulations.
    • Assessment of glass suitability for general-purpose and specialized recording techniques.

    Main Results:

    • Borosilicate glasses (e.g., 7760, 7052, 7040) are recommended for general single-channel and whole-cell recordings.

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  • Corning 8161 exhibits superior electrical and thermal properties but requires careful evaluation for leachable components.
  • High lead, low melting point glasses (e.g., 8161, KG-12) are optimal for perforated-patch whole-cell recordings.

    • Conclusions:

    • The choice of glass must be tailored to the specific cell type and recording methodology.
    • Further validation is necessary for each cell type to mitigate issues like leaching.
    • Specific glass formulations offer distinct advantages for advanced electrophysiological techniques.