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Updated: Jul 5, 2026

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Published on: October 8, 2008

Fabrication of patch pipets.

J L Rae¹, R A Levis

¹Mayo Foundation, Rochester, Minnesota, USA.

Current Protocols in Neuroscience

|April 23, 2008

Summary

This summary is machine-generated.

This guide details fabricating patch pipets for electrophysiological measurements, emphasizing glass selection and pipet geometry for optimal noise and dynamic performance in patch clamp techniques.

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Area of Science:

Electrophysiology
Biophysics
Neuroscience

Background:

Patch clamping is a crucial electrophysiological technique for studying ion channels.
Reliable patch pipet fabrication is essential for high-quality electrophysiological data.

Purpose of the Study:

To provide a comprehensive guide on fabricating patch pipets.
To detail procedures for optimizing pipet geometry and performance.

Main Methods:

Detailed parameters for glass selection.
Step-by-step protocols for pulling patch and whole-cell pipets.
Techniques for elastomer coating, fire polishing, filling, and testing pipets.

Main Results:

Established procedures for reliably fabricating patch pipets.

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Optimization strategies for pipet geometry, noise reduction, and dynamic performance.

Considerations for different patch clamp applications.

Conclusions:

Proper patch pipet fabrication is critical for successful electrophysiological recordings.
Adherence to detailed protocols ensures optimal pipet performance for various experimental needs.