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[Application of EITB in immunodiagnosis of cysticercosis]
Li-na Wang1, Ling-yun Ge, Feng Miao
1Department of Immunology, Weifang Medical College, Weifang 261042, China.
Objective:
To establish a sensitive and specific method EITB for the immunodiagnosis of cysticercosis.
Methods:
Two-dimensional electrophoresis was used to analyze and purify the cyst fluid antigen. Western blotting using the sera of patients with cysticercosis, hydatidosis, and other heteroserum was used to screen the specific antigens. EITB using the specific antigen was established and compared with ELISA. The detection effect of serum and blood on filter paper in EITB was also compared.
Results:
Two specific antigens with isoelectric point (pI) of 9.4 and Mr of 14000 and 16600 were obtained, respectively. EITB method based on these antigens was established with the sensitivity and specificity of 92.5% and 100%, respectively, significantly higher than that of ELISA. The sensitivity of serum and filter paper blood was similar in EITB.
Conclusion:
A sensitive and specific EITB method for immunodiagnosis of cysticercosis was established.
