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Array comparative genomic hybridization with cyanin cis-platinum-labeled DNAs
Anton K Raap1, Marja J M van der Burg, Jeroen Knijnenburg
1Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands. a.k.raap@lumc.nl
Biotechniques
|July 31, 2004
Summary
Cyanine (CyN) cis-platinum compounds offer a reliable method for labeling DNA in array comparative genomic hybridizations (arrayCGH). This fluorescent labeling technique provides results comparable to enzymatic methods for genomic analysis.
Area of Science:
- Biochemistry
- Molecular Biology
- Genomics
Background:
- Fluorescent cis-platinum compounds target guanine's N7 atom for nucleic acid labeling.
- Applications include fluorescence hybridization and genomic analysis.
Purpose of the Study:
- To evaluate cyanine (CyN) cis-platinum labeling for array comparative genomic hybridizations (arrayCGH).
- To assess the reproducibility and reliability of this labeling method.
Main Methods:
- Labeling DNA samples with CyN3- and CyN5-cis-platinum at approximately 1% nucleotide incorporation.
- Performing arrayCGH on mouse and human tumor samples.
- Comparing results with standard enzymatic labeling techniques.
Main Results:
- Achieved reproducible and reliable DNA labeling using CyN cis-platinum compounds.
- Generated arrayCGH signal-to-background ratios of 30-40.
- Obtained results comparable to enzymatic labeling in tumor sample analyses.
Conclusions:
- Cy-cis-platinum labeling is a viable and effective alternative to enzymatic labeling for arrayCGH.
- This method offers a reliable approach for genomic analysis and hybridization applications.