Related Experiment Video
Updated: Aug 23, 2026
"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
Published on: February 19, 2019
New serum biomarkers for detection of HBV-induced liver cirrhosis using SELDI protein chip technology
Xiao-Dong Zhu1, Wei-Hua Zhang, Cheng-Lin Li
1Department of Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Zhongguancun Beiyitiao, Beijing 100080, China.
Aim:
To find new serum biomarkers for liver cirrhosis (LC) in chronic carriers of hepatitis B virus (HBV).
Methods:
Surface enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry was used to discover biomarkers for differentiating HBV induced LC from non-cirrhotic cohorts. A training population of 25 patients with HBV-induced LC, 20 patients with HCC, and 25 closely age-matched healthy men, was studied.
Results:
Two biomarkers with M(r) 7 772 and 3 933 were detected in sera of non-cirrhotic cohorts, but not in patients with HBV-induced LC. A sensitivity of 80% for all LC patients, a specificity of 81.8% for all non-cirrhotic cohorts and a positive predictive value of 75% for the study population were obtained.
Conclusion:
These two serum biomarkers for HBV-induced LC might be used for diagnosis and assessment of disease progression.
