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Updated: Jul 17, 2026
Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance
Published on: July 28, 2023
Detection of Helicobacter pylori in water by direct PCR
J A Benson1, K A Fode-Vaughan, M L P Collins
1Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53201, USA.
Aims:
This paper demonstrates a rapid, simple method for the detection of Helicobacter pylori in water that eliminates the need for recovery of cells or DNA extraction prior to PCR.
Methods And Results:
Direct polymerase chain reaction (DPCR) with primers specific for H. pylori ureA (urease, subunit A) were used to detect H. pylori added to groundwater. DPCR also detected H. pylori in a naturally contaminated water sample.
Conclusions:
DPCR should provide an improved method to assess contamination of water by H. pylori.
Significance And Impact Of The Study:
This simple, rapid method for detection of H. pylori in water will provide an improved means to investigate the possible role of water as a disease vector.
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