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Implementation and evaluation of a detector for forward propagated second harmonic signals
Nuno Moreno1, José A Feijó, Guy Cox
1Instituto Gulbenkian de Ciência, Oeiras, Portugal.
Micron (Oxford, England : 1993)
|August 4, 2004
Summary
This study presents an optimized detection system for second harmonic generation (SHG) imaging. Placing a detector at the condenser
Area of Science:
- Non-linear optics
- Biophotonics
- Microscopy
Background:
- Second harmonic generation (SHG) is an emerging non-linear imaging technique.
- Its adaptability to commercial multi-photon microscopes makes it attractive for biological imaging.
- Understanding SHG's capabilities requires efficient detection methods.
Purpose of the Study:
- To describe a novel SHG detection implementation.
- To optimize the collection of forward-propagating SHG light.
- To evaluate the performance of the new detection system.
Main Methods:
- An SHG detection system was designed and implemented.
- A Hamamatsu H957-08 PMT was positioned at the back-focal plane of the condenser on an inverted Nikon TE300 microscope.
- The system was controlled using BioRad 1024MP electronics.
- Performance was evaluated using potassium dihydrogen phosphate (KH2PO4) crystals and collagen samples.
Main Results:
- The implemented detector setup at the condenser's back-focal plane efficiently collects forward-propagating SHG signals.
- This configuration offers significant advantages over traditional detector placements.
- Successful SHG signal detection was demonstrated on KH2PO4 crystals and collagen.
Conclusions:
- Positioning a detector at the condenser's back-focal plane provides a highly efficient SHG detection system.
- This method enhances the collection of second harmonic signals.
- The described implementation is advantageous for SHG imaging applications.