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Overexpressed transglutaminase 5 triggers cell death
B Cadot1, A Rufini, V Pietroni
1Biochemistry Laboratory, IDI-IRCCS, University of Rome Tor Vergata, Rome, Italy.
Amino Acids
|August 4, 2004
Summary
Overexpressing transglutaminase 5 (TG5) in cells induces cell death. TG5 contains GTP-binding domains, suggesting it may be regulated by GTP, similar to transglutaminase 2.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Biology
Background:
- Transglutaminases (TGs) are enzymes crucial for cellular processes like differentiation and tissue repair.
- Transglutaminase 5 (TG5), initially identified in skin keratinocytes, plays a role in skin differentiation.
Purpose of the Study:
- To investigate the functional consequences of intracellular overexpression of transglutaminase 5.
- To explore the potential regulatory mechanisms of transglutaminase 5.
Main Methods:
- Cell transfection with TG5 expression vectors.
- Enzymatic activity assessment using fluoresceincadaverine staining.
- Analysis of DNA content to detect hypodiploidy.
- Sequence analysis to identify conserved domains.
Main Results:
- Intracellular overexpression of TG5 led to cell death.
- Transfected cells exhibited TG5 enzymatic activity.
- Cell death was characterized by the formation of hypodiploid DNA content.
- Sequence analysis revealed GTP-binding domains in TG5, homologous to those in TG2.
Conclusions:
- Transglutaminase 5 can induce cell death upon intracellular overexpression.
- TG5 possesses GTP-binding domains, suggesting potential GTP-dependent regulation.
- Further research into TG5's role in cell death and its regulation by GTP is warranted.