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Updated: Aug 23, 2026
Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
Published on: September 15, 2017
Direct evidence of the existence of specific LPS binding sites on vascular endothelial cells
Jian Chen1, Xiang-dong Luo, Qin Luo
1Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. hhhcc@tmmu.com.cn
Objective:
To investigate the binding characteristics of endothelial cell (EC) with LPS free from the participation of serum factors.
Methods:
Laser confocal microscope was employed in the observation of the binding of EC with FITC-LPS. The KD and the binding sites of each EC were calculated by radioligand binding assay of receptors (RBA) using [(3)H]-LPS.
Results:
The binding of EC with LPS was saturable, time and concentration dependent and it could be competed with overdosed LPS of the same type. The fluorescence mainly distributed in cytoplasm, especially near the nucleus, which could also be stained.
Conclusions:
There might be some specific LPS binding sites existing on ECs and LPS could function intracellularily.
