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Updated: Aug 23, 2026

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022
Optimization of the biodegradation of naphthalene by a microorganism isolated from petroleum contaminated soil
1Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL, Canada, A1B 3X9. amartin@mun.ca
Abstract:
Response surface methodology (RSM) was used to optimise the process parameters to obtain maximum biodegradation of naphthalene. The factorial design employed, a face-centred cube, helped in identifying the combined best parameter conditions. Naphthalene-containing medium was inoculated with Pseudomonas fluorescens and incubated at different levels of agitation, pH, and temperature, and analyzed by Gas Chromatography-Mass Spectroscopy. As a result of the optimisation process, the process parameters found for maximum degradation of naphthalene by P. fluorescens were an agitation speed of 186 rpm, a pH of 7.3, and a temperature of 22.8 degrees C.
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