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Updated: Aug 19, 2026

Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
DNA ploidy and cell cycle analysis in breast cancer
Jeffrey S Ross1, Gerald P Linette, James Stec
1Department of Pathology and Laboratory Medicine, Albany Medical College, NY 12208, USA.
Abstract:
During the past 10 years there has been considerable interest in the application of new technologies to identify human malignancy and predict disease outcome. Markers of cell proliferation and the technologies of flow cytometry and image analysis for the determination of DNA total content in human tumor cells have been studied in breast cancer for 20 years. In this review, the uses and limitations of these technologies for the determination of ploidy status are discussed. This review also considers the prognostic significance and potential clinical utility of ploidy measurements, S phase calculation, and individual cell cycle regulatory biomarker expression levels.
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