Melatonin suppresses reactive oxygen species induced by UV irradiation in leukocytes
Tobias W Fischer1, Georg Scholz, Brunhilde Knöll
1Department of Dermatology and Allergology, Friedrich-Schiller-University Jena, Jena, Germany. tobias.fischer@derma.uni-jena.de
Abstract:
An investigation of the antioxidative UV protective effect of melatonin was performed in an in vitro irradiation model with leukocytes. Leukocytes were isolated from EDTA-treated whole blood and taken up in phosphate-buffered saline (PBS). Five of 10 aliquots were incubated with 2 mmol/L melatonin and 5 with PBS as a control. The samples were irradiated by UV light (280-360 nm, max: 310 nm) at doses between 75 and 300 mJ/cm(2) or left unirradiated. Radical formation was measured using the chemiluminescence technique. Staining with trypan blue was performed to assess cell viability. Melatonin significantly suppressed radical formation in cell solutions irradiated from 75 to 300 mJ/cm(2) (P
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