Related Experiment Video
Updated: Aug 7, 2026

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
Published on: December 30, 2016
The ab initio crystal structure solution of proteins by direct methods. VI. Complete phasing up to derivative
C Giacovazzo1, D Siliqi, J Gonzalez Platas
1Dipartimento Geomineralogico, Universita' di Bari, Italy. giacovazzo@area.ba.cnr.it
Abstract:
The procedure described in the papers I-V of this series [Giacovazzo, Siliqi & Ralph (1994). Acta Cryst. A50, 503-505; Giacovazzo, Siliqi & Spagna (1994). Acta Cryst. A50, 609-621; Giacovazzo, Siliqi & Zanotti (1995). Acta Cryst. A51, 177-188; Giacovazzo & Gonzalez Platas (1995). Acta Cryst. A51, 398-404; Giacovazzo, Siliqi & Gonzalez Platas (1995). Acta Cryst. A51, 811-820], aiming at estimating protein phases via a single heavy-atom derivative, has been improved so as to extend phase determination to all the reflections up to derivative resolution. The quality of the resulting electron-density maps is checked for a number of test strutures. Some of the maps are immediately interpretable, and some can be interpreted after some cycles of solvent flattening and/or histogram matching. The correlation with classical SIR techniques is also discussed.
Related Concept Videos
X-ray Crystallography
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
Protein Organization
The primary structure of a protein is its amino acid sequence.
Determination of Crystal Structures

