Preliminary crystallographic analysis of chloroperoxidase from Caldariomyces fumago

Chloroperoxidase from the fungus Caldariomyces fumago has been crystallized in two space groups, C222(1) and P2(1)2(1)2(1) both of which are suitable for high-resolution X-ray studies. Parent data sets have been obtained to 2.16 A in space group C222(1) and 2.00 A in space group P2(1)2(1)2(1). Heavy-atom derivatives have been obtained with both forms and electron-density maps calculated. The heme has been located and continuous electron density between the heme and protein clearly indicates the location of the proximal ligand.