[Rapid diagnosis of 21 trisomy syndrome by fluorescence quantitative polymerase chain reaction]
Cheng-xue Jing1, Xiang-zhi Xie, Qian Zhang
1Department of Pediatrics, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021 PR China. chengxuejing@yahoo.com.cn
Objective:
To establish a method of fluorescence quantitative PCR to detect 21 trisomy syndrome.
Methods:
At first, using one pair of primer to simultaneously amplify different fragments of two highly homologous genes of the human liver-type phosphofructokinase located on chromosome 21 (PFKL-CH21) and the human muscle-type phosphofructokinase located on chromosome 1 (PFKM-CH1). Then, staining the PCR products of these homologous genes with SYBR Green I, comparing the fluorescence intensities of the bands after electrophoresis, and analyzing the data.
Results:
The relative fluorescence intensity ratios of PFKL-CH21/PFKM-CH1 in 21 trisomy syndrome and normal individuals were 1.58+/-0.17 (mean+/-SD) and 1.00+/-0.05 (mean+/-SD), respectively; the difference between the two groups was highly significant.
Conclusion:
SYBR Green I fluorescence quantitative polymerase chain reaction is an acurate, rapid, safe and practical approach for the detection of 21 trisomy syndrome.
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