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The multi-mode polarization modulation spectrometer: part 1: simultaneous detection of absorption, turbidity, and
Tudor Arvinte1, Tam T T Bui, Ali A Dahab
1Section de Pharmacie, Universitéde Genève, Quai Ernest-Ansermet 30, CH-1211 Geneva 4, Switzerland.
Abstract:
Circular dichroism (CD) is an important spectroscopic technique for monitoring chirality and biological macromolecule conformation. However, during a CD measurement, absorbance, light scattering/turbidity, and fluorescence can also be detected. The simultaneous measurement of these different spectral features for a single sample is the basis of a multi-mode optical spectrometer. This allows time-efficient gathering of complementary information and provides a scheme to ensure that CD measurements are reliable. Aspects of circular polarization differential light scattering, pH, and temperature variation of a protein (antibody) solution are described. A procedure to help ensure that CD measurements are reliable is described.