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Published on: October 4, 2017
Lipopolysaccharide-mediated cell activation without rapid mobilization of cytosolic free calcium
Herbert Bosshart1, Michael Heinzelmann
1Department of Surgery, Zurich University Hospital, Sternwartstrasse 14, CH-8091 Zurich, Switzerland. herbert.bosshart@usz.ch
Abstract:
The involvement of cytosolic free calcium (Ca2+) as a second messenger in lipopolysaccharide (LPS)-activated cellular pathways remains controversial. Using human monocyte-like THP-1 cells stably transfected with glycosylphosphatidylinositol-anchored human CD14, we have monitored the concentration of intracellular Ca2+ after sequential addition of LPS, ATP, and ionomycin. Whereas ATP and ionomycin induced rapid elevations of cytosolic Ca2+, no such changes were observed after LPS addition. These data argue against the participation of cytosolic Ca2+ in the CD14-dependent LPS activation pathway.
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