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Updated: Aug 23, 2026

An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses
Published on: July 7, 2017
[Role of interleukin-9 in the pathogenesis of chronic obstructive pulmonary disease]
Zheng Liu1, Wan-zhen Yao, Yan Chen
1Department of Respiratory Medicine, Peking University Third Hospital, Beijing 100083, China.
Objective:
To study the role of interleukin-9 in the airway inflammation from patients with COPD.
Methods:
Induced sputum was obtained from 30 COPD patients with stable disease(group A),31 asthmatics patients with stable disease(group B) and 15 healthy individuals(group C). IL-9,IL-5 and IL-8 in sputum supernatants were measured by sandwich enzyme-linked immunosorbent assay(ELISA) and IL-9 positive expression and quantitative analysis were conducted by Streptavidin peroxidase method and image analysis technology.
Results:
The levels of IL-9 in group A and B were all significantly higher than those in groups C. IL-9 positive expression mainly located in the cytoplasm of macrophages. The positive rates of IL-9 in group A and B were all significantly higher than that of group C (chi(2)=20.821, 19.908, P<0.001 . The percentage of neutrophil and the level of IL-8 in group A was significantly higher than those of groups B and C. The level of IL-9 was positively correlated with the number of macrophages in group A(r=0.407,P=0.039).
Conclusion:
The level of IL-9 from the patients with COPD was significantly higher than that of healthy individuals and correlated with the number of macrophages and the level of IL-8. This suggests that IL-9 may be used as a new diagnostic marker in airway inflammation of COPD.
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