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Updated: Aug 23, 2026
Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
Published on: August 20, 2017
[Establishment of a single cell-cloned mouse embryonic stem cell line]
1Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100083, China. phmei@tsinghua.edu.cn
Objective:
To establish embryonic stem cells culture system and methods.
Methods:
Inner cell masses were isolated from blastocysts of 3.5 day-old 129SvJ mice by immunosurgery, seeded onto gamma-irradiated mouse fibroblasts feeder layer. The outgrowths were passed by digestion with trypsin. Surface markers were identified by cytochemistry and immunohistochemistry. Karyotype was tested with standard G-banding technique. Differential potency was tested both in vivo and in vitro.
Results:
One single cell cloned mouse embryonic stem cell line G(11) was established, which had normal 40XY karyotype and proliferated rigorously for a long time, expressed surface markers such as AP and SSEA-1, and was able to differentiate into many cell types both in vivo and in vitro.
Conclusion:
Mouse embryonic stem cell culture system has been established in our laboratory.
