Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A superoxide sensor based on a multilayer cytochrome c electrode.

Moritz K Beissenhirtz1, Frieder W Scheller, Fred Lisdat

  • 1Analytical Biochemistry, Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25, H. 25, 14476 Golm, Germany.

Analytical Chemistry
|August 17, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing analytical performance of tyrosinase-based sensors with nanoparticles for detection of isoproterenol.

Turkish journal of chemistry·2026
Same author

Quantum Dot/TiO<sub>2</sub> Nanocomposite-Based Photoelectrochemical Sensor for Enhanced H<sub>2</sub>O<sub>2</sub> Detection Applied for Cell Monitoring and Visualization.

Small (Weinheim an der Bergstrasse, Germany)·2024
Same author

A Strep-Tag Imprinted Polymer Platform for Heterogenous Bio(electro)catalysis.

Angewandte Chemie (International ed. in English)·2024
Same author

Specific features of epitope-MIPs and whole-protein MIPs as illustrated for AFP and RBD of SARS-CoV-2.

Mikrochimica acta·2024
Same author

Imprinted Polymers on the Route to Plastibodies for Biomacromolecules (MIPs), Viruses (VIPs), and Cells (CIPs).

Advances in biochemical engineering/biotechnology·2023
Same author

Correction: Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein.

Chemical science·2023
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
Same journal

Development of a Tapping-Mode Scanning Probe Electrospray Ionization Platform for High-Sensitivity and Long-Term Stability in Single-Cell Mass Spectrometry Imaging of Tissue.

Analytical chemistry·2026
Same journal

A Solid-State Near-Infrared Fluorescent Probe by a Synergistic Extended Conjugated System for Detecting Cys with Long-Term Imaging in Orthotopic Bladder Cancer.

Analytical chemistry·2026
Same journal

Air-Based Porous Array Dielectric Barrier Discharge Ionization Source for Explosive Trace Detection.

Analytical chemistry·2026
Same journal

PSAQ<sup>+1</sup>: Absolute Protein Quantification Using a <sup>13</sup>C<sub>1</sub>-Labeled Protein Standard, Coisolation of Peptide Pairs and LC-PRM.

Analytical chemistry·2026
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
See all related articles

This study introduces a novel multilayer cytochrome c electrode for superoxide radical quantification. Multilayer electrodes demonstrate enhanced sensitivity and stability for superoxide detection compared to traditional monolayer sensors.

Area of Science:

  • Electrochemistry
  • Biosensors
  • Materials Science

Background:

  • Superoxide radical is a key reactive oxygen species implicated in various physiological and pathological processes.
  • Accurate quantification of superoxide is crucial for understanding oxidative stress and developing therapeutic strategies.
  • Existing biosensors for superoxide often face limitations in sensitivity and stability.

Purpose of the Study:

  • To develop and characterize a novel multilayer cytochrome c electrode for sensitive and stable quantification of superoxide radicals.
  • To investigate the electrochemical properties and electron transfer mechanisms within the multilayer assembly.
  • To evaluate the performance of the developed electrode for in vitro superoxide detection.

Main Methods:

  • Fabrication of multilayer electrodes using alternating layers of cytochrome c and poly(aniline(sulfonic acid)) on a gold wire.

Related Experiment Videos

  • Surface Plasmon Resonance (SPR) for structural characterization.
  • Electrochemical techniques (cyclic voltammetry, impedance spectroscopy) for performance evaluation.
  • In vitro quantification of enzymatically generated superoxide.

    • Main Results:

    • Successful formation of multilayer structures with 2-15 protein layers exhibiting electrochemical communication.
    • Substantial increase in electrochemically active cytochrome c with increasing layers, over an order of magnitude higher for >10 layers compared to monolayers.
    • Demonstrated a protein-protein electron transfer mechanism within the multilayer assembly.
    • Multilayer electrodes showed superior sensitivity for superoxide quantification compared to monolayer sensors.
    • A 6-layer electrode achieved the highest sensitivity, a half-order of magnitude increase over monolayers.
    • Optimized stability through thermal treatment, maintaining sensor performance after multiple uses and storage.

    Conclusions:

    • The novel multilayer cytochrome c electrode offers significantly enhanced sensitivity and stability for superoxide quantification.
    • The protein-protein electron transfer model provides insight into the electrode's mechanism.
    • This advanced biosensor holds promise for improved diagnostics and research in oxidative stress-related conditions.