Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Conditional Reactivation of Lysozyme Nanosystems via Hydrophobic Ion Pairing.

ACS nanoscience Au·2026
Same author

Micropillar-Based Biosensor for Monitoring Contamination- and Compound-Induced Metabolic States in Cell Cultures.

ACS applied materials & interfaces·2026
Same author

NIR-II Light-Modulated Smart Drug Delivery System Utilizing Drug-Gated Nanocomposite Hydrogel for Boosting Anticancer Efficacy.

Polymer science & technology (Washington, D.C.)·2026
Same author

Towards targeted drugs and next generation of nanomedicines.

Beilstein journal of nanotechnology·2026
Same author

Uptake of Nanoparticles as a Model System for Viruses under the Presence of Chloroquine.

ACS nanoscience Au·2026
Same author

Poking Pluripotency: Nanoinjection Into Human iPSCs.

Advanced materials (Deerfield Beach, Fla.)·2026

Related Experiment Video

Updated: Jun 14, 2025

Preparation and Use of Photocatalytically Active Segmented Ag|ZnO and Coaxial TiO2-Ag Nanowires Made by Templated Electrodeposition
12:47

Preparation and Use of Photocatalytically Active Segmented Ag|ZnO and Coaxial TiO2-Ag Nanowires Made by Templated Electrodeposition

Published on: May 2, 2014

21.7K

Quantum Dot/TiO2 Nanocomposite-Based Photoelectrochemical Sensor for Enhanced H2O2 Detection Applied for Cell

Shuang Zhao1,2, Zhao Yue3, Dingcheng Zhu1,4

  • 1Fachbereich Physik, CHyN, Universität Hamburg, Hamburg, 22761, Germany.

Small (Weinheim an Der Bergstrasse, Germany)
|August 30, 2024
PubMed
Summary

This study introduces novel quantum dot (QD) electrodes for monitoring living cell metabolism via photoelectrochemical (PEC) measurements. The enhanced QD-TiO2 heterojunction improves signal quality and enables sensitive hydrogen peroxide detection for cell activity monitoring.

Keywords:
biocompatible interface with atomic layer depositionbiosensordetection of cellular metabolismlight addressable potentiometric sensorphotocurrent measurements

More Related Videos

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.2K
Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions
10:21

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions

Published on: October 5, 2019

8.3K

Related Experiment Videos

Last Updated: Jun 14, 2025

Preparation and Use of Photocatalytically Active Segmented Ag|ZnO and Coaxial TiO2-Ag Nanowires Made by Templated Electrodeposition
12:47

Preparation and Use of Photocatalytically Active Segmented Ag|ZnO and Coaxial TiO2-Ag Nanowires Made by Templated Electrodeposition

Published on: May 2, 2014

21.7K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.2K
Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions
10:21

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions

Published on: October 5, 2019

8.3K

Area of Science:

  • Nanotechnology
  • Electrochemistry
  • Biomedical Engineering

Background:

  • Photoelectrochemical (PEC) sensors offer promising avenues for real-time biological monitoring.
  • Quantum dots (QDs) exhibit unique optical and electronic properties suitable for biosensing applications.
  • Improving the stability, signal-to-noise ratio, and biocompatibility of QD-based sensors is crucial for effective cell metabolism monitoring.

Purpose of the Study:

  • To develop and optimize a quantum dot (QD)-electrode for monitoring living cell metabolism using photoelectrochemical (PEC) measurements.
  • To enhance the performance of PEC sensors through the introduction of a QD-TiO2 heterojunction.
  • To demonstrate the utility of the optimized sensor for hydrogen peroxide (H2O2) detection and direct monitoring of cell activity.

Main Methods:

  • Fabrication of QD-electrodes utilizing CdSe/ZnS quantum dots (QDs) and TiO2.
  • Integration of a QD-TiO2 heterojunction using atomic layer deposition (ALD) to improve charge carrier dynamics.
  • Optimization of TiO2 layer thickness and QD immobilization strategies for enhanced photocurrent generation.
  • Characterization of the sensor's performance for hydrogen peroxide (H2O2) sensing and cell activity monitoring.

Main Results:

  • The QD-TiO2 heterojunction significantly enhanced photocurrent signals (over an order of magnitude) and improved sensor stability and signal-to-noise ratio.
  • Optimal TiO2 thickness (approximately 5 nm) was determined, leading to substantial improvements in PEC performance.
  • The developed sensor demonstrated high sensitivity for hydrogen peroxide (H2O2) detection down to µmolar concentrations, with excellent reusability, stability, response rate, and repeatability.
  • The system successfully monitored the activity of living cells cultured directly on the sensor surface.

Conclusions:

  • The optimized QD-TiO2 heterojunction electrode represents a significant advancement in developing highly sensitive and stable PEC sensors for biological applications.
  • This technology enables effective monitoring of cellular metabolism and activity through sensitive detection of key biomarkers like H2O2.
  • The enhanced biocompatibility and performance of the sensor pave the way for sophisticated in-situ cellular analysis and diagnostics.