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PEDOT:PSS - A suitable interface for the enzyme PQQ-GDH on electrodes
1Biosystems Technology, Technical University of Applied Sciences Wildau, 15745 Wildau, Germany.
Bioelectrochemistry (Amsterdam, Netherlands)
|July 18, 2026
Summary
We developed a simple method for creating enzyme electrodes using PEDOT:PSS for mediator-free glucose detection. This biosensor platform shows stable and efficient direct electron transfer for glucose dehydrogenase (GDH).
Area of Science:
- Electrochemistry
- Biosensors
- Materials Science
Background:
- Enzyme immobilization on electrodes is crucial for biosensing and bioelectrocatalysis.
- Conducting polymers like PEDOT:PSS offer promising platforms for enzyme electrode development.
Purpose of the Study:
- To present a minimalistic manufacturing strategy for enzyme electrodes.
- To demonstrate mediator-free, oxygen-independent glucose detection using PQQ-GDH immobilized on PEDOT:PSS.
- To investigate the performance and stability of the developed enzyme electrode.
Main Methods:
- Fabrication of enzyme electrodes by depositing PEDOT:PSS on gold electrodes.
- Adsorptive immobilization of pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH).
- Electrochemical characterization including cyclic voltammetry and faradaic impedance spectroscopy (f-EIS).
- Analysis of glucose concentration-dependent activity and pH stability.
Main Results:
- Significant catalytic currents were observed for glucose detection.
- An apparent Km-value of 0.16 ± 0.03 mM was determined.
- The enzyme electrode exhibited stability over a pH range of 5.5–7.2.
- Faradaic impedance spectroscopy successfully monitored enzymatic activity.
Conclusions:
- PEDOT:PSS is an excellent platform for mediator-free bioelectrocatalysis using PQQ-GDH.
- Direct electron transfer (DET) between the enzyme and the electrode was confirmed.
- The developed enzyme electrode shows potential for advanced biosensing applications, including flexible electronics.
