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[Gene expression profile in early differentiation of K562 cells induced by matrine]
Yan Zhang1, Ling-Di Ma, Yu-Juan He
1Department of Clinical Biochemistry, Chongqing University of Medical Sciences, Chongqing 400016, China.
Objective:
To compare gene expression profile in K562 cells induced by matrine, so as to screen for differentiation related genes.
Methods:
K562 cells were exposed to 0.1 mg/ml matrine for 3 hours, and gene expression profiles in matrine-treated and non-treated cells were studied by cDNA microarray.
Results:
From 8465 screened genes, 30 differentially expressed genes were found. Among them 18 showed decreased expression including oncogene and proto-oncogene, signal transduction gene, DNA binding and transcription gene etc, 12 showed increased expression including cell receptor gene, immune-associated gene, metabolism-associated gene etc in matrine-treated K562 cells as compared with that in non-treated cell.
Conclusion:
The genes, that are closely associated with differentiation of can-cer cell, could be the potential targets for cancer treatment.
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