Sonochemically fabricated microelectrode arrays for biosensors--part II. Modification with a polysiloxane coating

Area of Science:

• Electrochemistry
• Materials Science
• Biosensors

Background:

• Development of high-density microelectrode arrays is crucial for sensitive biosensing applications.
• Glucose oxidase enzymes are widely used in electrochemical sensors for glucose monitoring.
• Sonochemical fabrication offers a unique method for creating microstructures.

Purpose of the Study:

• To develop a polymer-modified glucose oxidase microelectrode array with high microelectrode population density.
• To investigate the fabrication process using sonochemistry and electropolymerization.
• To characterize the physical and electrochemical properties of the fabricated sensor.

Main Methods:

• Fabrication of microelectrode array using sonochemical treatment of an insulating film on screen-printed electrodes.
• Electropolymerization of aniline to form polyaniline protrusions containing glucose oxidase.
• Deposition of a polysiloxane protective coating for diffusion control.
• Characterization of physical and electrochemical properties.

Main Results:

• Successful fabrication of a polymer-modified glucose oxidase microelectrode array with densities up to 2.5 x 10(5) microelectrodes/cm(2).
• Polyaniline protrusions effectively entrapped glucose oxidase within microelectrode cavities.
• Polysiloxane coating provided protection and controlled mass transport.
• Demonstrated performance of the final glucose oxidase-based microelectrode sensor array.

Conclusions:

• Sonochemical fabrication combined with electropolymerization is an effective method for creating high-density glucose oxidase microelectrode arrays.
• The developed sensor exhibits promising characteristics for glucose detection.
• The polymer modification and protective coating enhance sensor performance and stability.