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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

Group B rotaviruses similar to strain CAL-1, have been circulating in Western India since 1993

S D Kelkar1, J K Zade

  • 1Rotavirus Department, National Institute of Virology, Pune, India.

Epidemiology and Infection
|August 18, 2004
PubMed

Insights

Group B rotavirus, a cause of severe adult diarrhea, was detected in Pune, India, circulating since 1993. Further surveillance is crucial for this emerging public health threat.

Area of Science:

  • Virology
  • Gastroenterology
  • Epidemiology

Background:

  • Group A rotaviruses are common causes of pediatric diarrhea.
  • Group B rotavirus (ADRV) caused major epidemics in China and was later detected in India.
  • Human group B rotavirus is rarely reported outside China, highlighting the significance of new detections.

Purpose of the Study:

  • To investigate the presence of non-Group A rotaviruses in adult diarrhea cases in Pune, India.
  • To identify and characterize Group B rotavirus strains circulating in the region.
  • To assess the potential public health impact of emerging Group B rotavirus infections in adults.

Main Methods:

  • Faecal specimens from hospitalized adult diarrhea patients negative for Group A rotavirus were analyzed using polyacrylamide gel electrophoresis (PAGE).
  • Suspected Group B rotavirus samples were further tested using reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR.
  • Sequencing of PCR products was performed to compare with known Group B rotavirus strains.

Main Results:

  • Three adult faecal specimens collected in 1993 and 1998 showed rotavirus RNA migration patterns consistent with Group B rotavirus.
  • RT-PCR and nested PCR confirmed the presence of Group B rotavirus in these specimens.
  • Sequence analysis revealed the highest identity with the CAL-1 strain reported from Kolkata, India.

Conclusions:

  • Human Group B rotaviruses have been circulating in Pune, India, since at least 1993.
  • This emerging virus may cause severe disease in adults in India, necessitating increased surveillance.
  • Seroepidemiological studies in humans and animals in Western Maharashtra are recommended to understand the virus's spread.

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