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Updated: Aug 23, 2026
Isolation and Primary Culture of Rat Hepatic Cells
Published on: June 29, 2012
Expression of vascular endothelial growth factor gene in primary cultured rat hepatocytes
Jin-Lin Wang1, Jun Ming, Xiao-Dong Zhou
1Department of Hepatobiliary Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China. wangjinlin@hotmail.com
Background:
It is the key point for vascular endothelial growth factor (VEGF121) gene related therapy as to how to transfect and express the gene safely, effectively and repeatedly. This study was designed to investigate the VEGF121 transfection and expression in primary cultured rat hepatocyte.
Methods:
After construction of vector internal ribosome entry site-enhanced yellow fluorescent protein (pIRES-EYFP)/VEGF121, the transfection and expression of the exogenous VEGF121 gene in primary cultured rat hepatocytes were observed through RT-PCR, Western blot and fluorescent microscopy.
Results:
pIRES-EYFP/VEGF121 plasmid was constructed and transfected successfully into primary cultured rat hepatocytes, the transfection and expression of gene in primary cultured rat hepatocytes were examined by RT-PCR and Western blot, and yellow-green fluorescence was observed through a fluorescent microscope.
Conclusion:
The successful transfection and expression of plasmid pIRES-EYFP/VEGF121 in primary cultured rat hepatocytes provides a foundation for hepatocyte transplantation and gene therapy after modification of hepatocytes by the gene.
