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Related Experiment Videos

Multiple effects of DiS-C3(5) on mitochondrial structure and function.

Takenori Yamamoto1, Aiko Tachikawa, Satsuki Terauchi

  • 1Institute for Genome Research, Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.

European Journal of Biochemistry
|August 20, 2004
PubMed
Summary

3,3'-Dipropyl-2,2'-thiadicarbocyanine iodide (DiS-C(3)(5)) affects isolated mitochondria differently based on concentration and conditions. It can induce mitochondrial permeability transition and cytochrome c release, or inhibit calcium-induced PT.

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Area of Science:

  • Mitochondrial Physiology
  • Biochemistry
  • Cell Biology

Background:

  • 3,3 '-Dipropyl-2,2 '-thiadicarbocyanine iodide (DiS-C(3)(5)) is a common tracer for mitochondrial membrane potential.
  • Previous studies indicated its inhibitory effect on NADH-driven mitochondrial electron transfer.

Purpose of the Study:

  • To investigate the detailed effects of DiS-C(3)(5) on the structure and function of isolated mitochondria.
  • To elucidate the mechanisms underlying DiS-C(3)(5) 's dose-dependent actions.

Main Methods:

  • Isolated mitochondria were treated with varying concentrations of DiS-C(3)(5).
  • Mitochondrial oxygen consumption was measured.
  • Mitochondrial membrane permeability to poly(ethylene glycol) was assessed.
  • Mitochondrial configuration was analyzed using transmission electron microscopy.

Related Experiment Videos

  • Cytochrome c release was monitored.

    • Main Results:

    • DiS-C(3)(5) exhibited biphasic effects on succinate-energized mitochondria in the presence of inorganic phosphate.
    • High concentrations (50 μM) accelerated oxygen consumption by inducing mitochondrial permeability transition (PT) and releasing cytochrome c.
    • Low concentrations (5 μM) inhibited latent oxygen consumption by preventing Ca(2+)-induced PT.
    • In the absence of inorganic phosphate, DiS-C(3)(5) induced mitochondrial swelling and membrane changes without cytochrome c release.

    Conclusions:

    • DiS-C(3)(5) can induce mitochondrial permeability transition in a dose-dependent manner.
    • The dye's effects are modulated by the presence of inorganic phosphate and calcium ions.
    • DiS-C(3)(5) serves as a versatile tool for studying mitochondrial PT and its consequences, including cytochrome c release.