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Related Experiment Videos

Gene expression variation between mouse inbred strains.

Rolf Turk1, Peter A C 't Hoen, Ellen Sterrenburg

  • 1Center for Human and Clinical Genetics, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, Nederland. r.turk@lumc.nl

BMC Genomics
|August 20, 2004
PubMed
Summary
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Genetic background significantly impacts gene expression profiles in mouse hindlimb muscle. Analyzing these genetic variations helps refine disease-related gene expression studies in animal models.

Area of Science:

  • Genomics
  • Molecular Biology
  • Animal Models

Background:

  • Investigated the influence of genetic background on gene expression.
  • Focused on mouse hindlimb muscle transcriptomes from five inbred strains.

Purpose of the Study:

  • To quantify the effect of genetic background on gene expression profiles.
  • To establish a method for accounting for genetic variation in expression studies.

Main Methods:

  • Transcriptome analysis using spotted oligonucleotide microarrays.
  • Statistical analysis including ANOVA with false discovery rate control.
  • Validation of differential gene expression using quantitative RT-PCR.

Main Results:

  • 1.4% of analyzed genes showed significant differential expression between mouse strains.

Related Experiment Videos

  • Genetic background effects were approximately ten-fold lower than dystrophic defect effects.
  • Differential expression was confirmed for several genes.

    • Conclusions:

    • Evaluating genetic background effects is crucial for comparing expression patterns in diverse animal models.
    • Excluding background-affected genes enhances analysis of disease-related expression changes.
    • This approach is more efficient than traditional methods like backcrossing for isogenic backgrounds.