Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Adenovirus vector-mediated doxycycline-inducible RNA interference.

Tetsuji Hosono1, Hiroyuki Mizuguchi, Kazufumi Katayama

  • 1Division of Cellular and Gene Therapy Products, National Institute of Health Sciences, Tokyo 158-8501, Japan.

Human Gene Therapy
|August 21, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vaccine Ligand Binding Assay Life Cycle Management, Assay Maintenance, Monitoring and Transfer.

Bioanalysis·2026
Same author

Efficacy of intranasal adenovirus vector vaccine is attenuated by type I IFN-induced NK cell activation.

Molecular therapy. Advances·2026
Same author

Correction to "Linker and Conjugation Site Synergy in Antibody-Drug Conjugates: Impacts on Biological Activity".

Bioconjugate chemistry·2026
Same author

[Regulatory Science Study for Ensuring the Quality of Therapeutic extracellular vesicle（EV） Products Including Exosomes and Promoting Their Development].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
Same author

Effects of cellular receptors on the vaccine efficacies of adenovirus vector-based vaccines following intramuscular and intranasal administration.

Scientific reports·2026
Same author

Liver-Specific Suppression of PLA2G6/iPLA<sub>2</sub>β Improves Glucose and Lipid Metabolism in High-Fat Diet-Fed Mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Researchers developed an adenovirus (Ad) vector system for doxycycline (Dox)-inducible RNA interference (RNAi) to control gene expression. This system effectively silenced p53 and c-Myc in human cancer cells, offering a versatile tool for research and therapy.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Biotechnology

Background:

  • RNA interference (RNAi) is a key mechanism for gene silencing.
  • Adenovirus (Ad) vectors are efficient tools for gene delivery in vitro and in vivo.
  • Controlling gene knockdown with precision is crucial for research and therapeutic applications.

Purpose of the Study:

  • To develop a doxycycline (Dox)-inducible small interfering RNA (siRNA) expression system using adenovirus vectors.
  • To demonstrate the system's ability to regulate the expression of p53 and c-Myc in human cancer cells.
  • To evaluate the system's potential for gene function analysis and RNAi-based therapeutics.

Main Methods:

  • Construction of Ad vectors encoding a Dox-inducible H1 promoter-driven siRNA expression cassette.

Related Experiment Videos

  • Coinfection of human cancer cells with Ad vectors expressing the siRNA system and a tetracycline repressor.
  • Administration of doxycycline (Dox) to induce siRNA expression and subsequent gene knockdown.
  • Quantification of p53 and c-Myc expression levels via dose-response studies.

    • Main Results:

    • Adenovirus vector-mediated delivery of the Dox-inducible siRNA system resulted in significant knockdown of p53 and c-Myc expression.
    • Gene silencing was observed in a dose-dependent manner, correlating with both Dox concentration and viral vector dose.
    • Regulated and controllable silencing of target gene expression was successfully achieved.

    Conclusions:

    • The developed Ad vector-mediated inducible RNAi system provides efficient and controllable gene silencing.
    • This system demonstrates broad applicability for various cell types, both in vitro and in vivo.
    • The ability to modulate gene knockdown by Dox dose makes it a valuable tool for gene function studies and potential RNAi therapeutics.