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In Vitro Fertilization

In Vitro Fertilization

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The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

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Related Experiment Video

Updated: Jul 22, 2026

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

Published on: August 5, 2011

Assisted hatching improves implantation rates on cryopreserved-thawed embryos. A randomized prospective study.

A Gabrielsen¹, I Agerholm, B Toft

¹Ciconia Fertility Clinic, Aarhus, DK-8270 Højbjerg, Denmark. ag@ciconia.dk

Human Reproduction (Oxford, England)

|August 21, 2004

Summary

Assisted hatching using acidic Tyrode's solution significantly improved implantation rates for cryopreserved-thawed embryos. This technique enhances the chances of successful pregnancy following embryo transfer.

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Last Updated: Jul 22, 2026

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Area of Science:

Reproductive Medicine
Embryology
In Vitro Fertilization

Background:

Cryopreserved-thawed embryos exhibit lower pregnancy rates than fresh embryos.
Zona pellucida hardening in cryopreserved embryos may impede implantation.
Limited research exists on assisted hatching for cryopreserved embryos.

Purpose of the Study:

To evaluate the efficacy of assisted hatching on the implantation rate of cryopreserved-thawed day 2 embryos.
To determine if acidic Tyrode's solution improves outcomes in assisted hatching procedures.
To compare implantation and pregnancy rates between assisted hatching and control groups.

Main Methods:

A prospective randomized study involving 253 cryopreserved-thawed embryo cycles.
Embryos were assigned to assisted hatching or a control group using pseudorandomization.
Assisted hatching was performed using acidic Tyrode's solution one hour before embryo transfer.

Main Results:

The implantation rate was significantly higher in the assisted hatching group (11.4%) compared to the control group (5.8%) (P<0.05).
No significant difference was observed in clinical pregnancy or positive serum HCG rates between the groups.
Age did not show a significant impact on outcomes, though few women over 38 were included.

Conclusions:

Assisted hatching with acidic Tyrode's solution effectively increases the implantation rate of cryopreserved-thawed embryos.
This method presents a viable strategy to improve IVF success with cryopreserved embryos.
Further research may explore optimal application across different patient demographics.