Related Experiment Videos
Interaction-dependent gene expression in Mla-specified response to barley powdery mildew.
Rico A Caldo1, Dan Nettleton, Roger P Wise
1Department of Plant Pathology and Center for Plant Responses to Environmental Stresses, Iowa State University, Ames, Iowa 50011-1020, USA.
The Plant Cell
|August 21, 2004
Summary
Barley plants
Area of Science:
- Plant pathology
- Molecular biology
- Genomics
Background:
- Plant recognition of pathogen molecules shapes host-microbe interactions.
- Understanding host gene expression during pathogen invasion is crucial.
Purpose of the Study:
- To analyze global host gene expression in barley during biotrophic pathogen invasion.
- To investigate gene expression patterns in barley-Blumeria graminis interactions.
Main Methods:
- Analysis of mRNA abundance for 22,792 barley genes across 36 genotype x pathogen x time interactions.
- Utilized a split-split-plot design with near-isogenic barley lines and specific Blumeria graminis f. sp hordei isolates.
- Employed linear mixed model analysis to identify differentially expressed genes (P < 0.0001).
Main Results:
- 22 host genes showed similar upregulation up to 16 hours post-inoculation (hai) in all interactions.
- Significant divergent expression observed from 16 to 32 hai, with transcript suppression in compatible interactions.
- Identified a link between general/specific pathogen molecule recognition and gene-for-gene resistance.
Conclusions:
- Host gene expression diverges during barley-powdery mildew interactions.
- Findings support the hypothesis that host-specific resistance evolved from preventing pathogen suppression of basal defense.