T cell subset-specific expression of antigen receptor beta chains in alpha chain-transgenic mice

A large number of V beta 8 gene-encoded cDNA were analyzed from peripheral CD4+ and CD8+ T cell subsets of T cell receptor (TcR) alpha chain-transgenic mice. This analysis demonstrates that a limited repertoire of TcR beta chains are co-expressed with the transgenic alpha chain. Most importantly, certain V beta 8-J beta combinations were found exclusively in one of the subsets and, in some cases, subset-specific differences were localized to the VDJ junctional region of the beta chain genes. In contrast, CD4-CD8- transgenic T cells, as well as CD4+ and CD8+ T cells from normal littermate controls, were found to express diverse beta chain repertoires. The present study suggests that beta chains with distinct structural characteristics are expressed in the CD4+ and CD8+ subsets, respectively. Moreover, the data suggest that the same structural constraints do not apply to the population of CD4-CD8- transgenic T cells.