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Updated: Aug 22, 2026
Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics
Published on: March 1, 2022
[Study on biological behavior of osteoblast and vascular endothelial cell culture]
Gang Rui1, Zhesheng Wang, Gang Lu
1Department of Orthopedic Surgery, the First Affiliated Hospital, China Medical University, Shenyang Liaoning, 110001, PR China.
Objective:
To study the biological behavior of osteoblast and vascular endothelial cell culture.
Methods:
The osteoblasts and vascular endothelial cells were obtained from calvarial bone and renal cortex of 2-week rabbits respectively. The experiment were divided into group A (osteoblasts), group B (vascular endothelial cells) and group C (co-cultured osteoblasts and vascular endothelial cells). The cells were identified with cytoimmunochemical staining. The cellular biological behavior and compatibility were observed under inverted phase contrast microscope and with histological staining. The cells viability and alkaline phosphatase (ALP) activity were measured.
Results:
The cytoimmunochemical staining showed that the cultured cells were osteoblasts and vascular endothelial cells. The cellular compatibility of osteoblasts and vascular endothelial cells was good. The ALP activity was higher in group C than in group A and group B (P<0.01), and it was higher in group A than in group B (P<0.05). In group C, the cell proliferation were increased slowly early, but fast later.
Conclusion:
The cellular compatibility of osteoblasts and vascular endothelial cells were good. The vascular endothelial cells can significantly increased the osteoblast viability and ALP activity, and the combined cultured cells have greater proliferation ability.
