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Updated: Aug 22, 2026

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
Published on: March 31, 2022
DNA strand breaks induced by 0-4 eV electrons: the role of shape resonances
Frédéric Martin1, Paul D Burrow, Zhongli Cai
1Faculty of Medicine, Université de Sherbrooke, Quebec, J1H 5N4, Canada.
Abstract:
Collisions of 0-4 eV electrons with thin DNA films are shown to produce single strand breaks. The yield is sharply structured as a function of electron energy and indicates the involvement of pi* shape resonances in the bond breaking process. The cross sections are comparable in magnitude to those observed in other compounds in the gas phase in which pi* electrons are transferred through the molecule to break a remote bond. The results therefore support aspects of a theoretical study by Barrios et al. [J. Phys. B 106, 7991 (2002)]] indicating that such a mechanism could produce strand breaks in DNA.
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