Related Experiment Video
Updated: Jul 6, 2026
13:56
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
Published on: July 18, 2013
Interaction between human cyclin A and adenovirus E1A-associated p107 protein
Science (New York, N.Y.)
|January 3, 1992
Summary
Adenovirus E1A oncoproteins interact with cellular proteins like pRB and p107. New findings show cyclin A binds p107, linking cell cycle control to p107 function.
Area of Science:
- Molecular Biology
- Virology
- Cell Biology
Background:
- Adenovirus early region 1A (E1A) gene products are potent oncoproteins.
- E1A-induced cellular changes are mediated through interactions with cellular proteins.
- Key E1A-interacting proteins include retinoblastoma protein (pRB), p107, cyclin A, and p33cdk2.
Purpose of the Study:
- To investigate the interactions between E1A-associated cellular proteins.
- To determine the relationship between p107 and cell cycle regulators like cyclin A.
- To elucidate the functional implications of these protein interactions.
Main Methods:
- Co-immunoprecipitation assays to study protein binding.
- Analysis of protein interactions in the presence and absence of E1A.
- Biochemical characterization of protein complexes.
Main Results:
- pRB and p107 are structurally related and bind to both E1A and SV40 large T oncoprotein.
- Cyclin A and p33cdk2 form a kinase complex involved in cell cycle control.
- Cyclin A directly binds to p107 independently of E1A.
Conclusions:
- The direct association of p107 with cyclin A establishes a link between p107 function and cell cycle regulation.
- These findings provide new insights into the mechanisms by which viral oncoproteins subvert cellular processes.
- Understanding these interactions is crucial for deciphering viral oncogenesis and cellular transformation.
Related Concept Videos
02:38The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...
01:09Epistasis Analysis
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
01:28Amebiasis
Entamoeba histolytica, a protozoan parasite, is responsible for intestinal and extraintestinal amebiasis. Though a significant proportion of infections remain asymptomatic, approximately 50 million individuals annually are estimated to present with clinical disease, resulting in up to 100,000 deaths globally. The disease burden is disproportionately high in regions with lower socioeconomic status, such as parts of India, Africa, Mexico, and Latin America.Etiology and TransmissionThe infective...