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A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
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Published on: July 18, 2013

Interaction between human cyclin A and adenovirus E1A-associated p107 protein

B Faha1, M E Ewen, L H Tsai

  • 1Massachusetts General Hospital Cancer Center, Charlestown 02129.

Science (New York, N.Y.)
|January 3, 1992
PubMed

Insights

Adenovirus E1A oncoproteins interact with cellular proteins like pRB and p107. New findings show cyclin A binds p107, linking cell cycle control to p107 function.

Area of Science:

  • Molecular Biology
  • Virology
  • Cell Biology

Background:

  • Adenovirus early region 1A (E1A) gene products are potent oncoproteins.
  • E1A-induced cellular changes are mediated through interactions with cellular proteins.
  • Key E1A-interacting proteins include retinoblastoma protein (pRB), p107, cyclin A, and p33cdk2.

Purpose of the Study:

  • To investigate the interactions between E1A-associated cellular proteins.
  • To determine the relationship between p107 and cell cycle regulators like cyclin A.
  • To elucidate the functional implications of these protein interactions.

Main Methods:

  • Co-immunoprecipitation assays to study protein binding.
  • Analysis of protein interactions in the presence and absence of E1A.
  • Biochemical characterization of protein complexes.

Main Results:

  • pRB and p107 are structurally related and bind to both E1A and SV40 large T oncoprotein.
  • Cyclin A and p33cdk2 form a kinase complex involved in cell cycle control.
  • Cyclin A directly binds to p107 independently of E1A.

Conclusions:

  • The direct association of p107 with cyclin A establishes a link between p107 function and cell cycle regulation.
  • These findings provide new insights into the mechanisms by which viral oncoproteins subvert cellular processes.
  • Understanding these interactions is crucial for deciphering viral oncogenesis and cellular transformation.

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