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Antibody to serum amyloid A.
Barbara J Rosenau1, Peter H Schur
1Division of Rheumatology/Immunology/Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Journal of Autoimmunity
|August 25, 2004
Summary
Antibodies to Serum amyloid A (SAA) were detected in some patients, with associations found in inflammatory cardiovascular diseases. This study introduces a new marker for potential disease association.
Area of Science:
- Immunology
- Clinical Chemistry
Background:
- Serum amyloid A (SAA) is a major acute phase reactant elevated during inflammation.
- Antibodies to C-reactive protein (CRP) are known, but antibodies to SAA have not been previously described.
- The study aimed to investigate the presence and clinical associations of anti-SAA antibodies.
Purpose of the Study:
- To develop and validate an ELISA for detecting antibodies to SAA.
- To determine if anti-SAA antibodies are present in patient populations.
- To explore associations between anti-SAA antibodies and various diseases, particularly inflammatory conditions.
Main Methods:
- An ELISA was developed to detect anti-SAA antibodies.
- Specificity was confirmed through inhibition assays.
- Serum samples from 138 patients and 62 healthy controls were analyzed.
- Patient charts were reviewed for clinical data and inflammation markers (CRP).
Main Results:
- A cutoff for elevated anti-SAA levels was established at 0.988.
- Elevated anti-SAA antibodies were found in 24 patients and 1 normal individual.
- Associations were observed between elevated anti-SAA levels and aortic stenosis, deep vein thrombosis, systemic lupus erythematosus, seizures, stroke, and atrial fibrillation.
- No correlation was found between anti-SAA and CRP levels.
Conclusions:
- Antibodies to SAA can develop in a subset of patients.
- A potential association exists between anti-SAA antibodies and inflammatory cardiovascular diseases.