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Interlaboratory comparison trial on cylindrospermopsin measurement.
Andrea Törökné1, Maria Asztalos, Maria Bánkiné
1Department of Hygienic Water Biology, National Institute of Environmental Health, P.O. Box 26, Budapest H-1450, Hungary. toroka@okk.antsz.hu
Analytical Biochemistry
|August 25, 2004
Summary
This study evaluated methods for measuring the cyanotoxin cylindrospermopsin (CYN) in cyanobacterial cells. The most effective extraction used 5% formic acid for accurate high-performance liquid chromatography (HPLC) analysis.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Toxicology
Background:
- Cylindrospermopsin (CYN) is a potent hepatotoxin produced by cyanobacteria.
- CYN inhibits protein synthesis and is found in freshwater blooms globally.
- Accurate measurement of CYN is crucial for public health and environmental monitoring.
Purpose of the Study:
- To evaluate the measurement of CYN in lyophilized cyanobacterial cells through an interlaboratory comparison.
- To assess the effectiveness of different extraction and high-performance liquid chromatography (HPLC) methods for CYN analysis.
Main Methods:
- An interlaboratory comparison involving six laboratories from Europe, Israel, and Australia.
- Extraction of CYN from lyophilized cyanobacterial cells.
- Analysis of CYN using high-performance liquid chromatography (HPLC).
- Statistical evaluation of methods based on ISO standards 5725-1 and -2.
Main Results:
- All participating laboratories demonstrated satisfactory performance in CYN extraction and HPLC analysis.
- The most effective extraction procedure involved using 5% formic acid.
- This method prevented interference in chromatograms during HPLC analysis with a specific mobile phase.
Conclusions:
- Standardized methods for CYN measurement are feasible and reliable.
- 5% formic acid is recommended for optimal CYN extraction prior to HPLC analysis.
- This contributes to accurate cyanotoxin monitoring in environmental samples.