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Technical note: Vaginal absorption of 1,25(OH)2D3 in cattle
N Okura1, N Yamagishi, Y Naito
1Large Animal Clinical Center, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. n-ohkura@mvg.biglobe.ne.jp
Journal of Dairy Science
|August 26, 2004
Summary
Intravaginal administration of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) in dairy cows demonstrated effective absorption and potential for preventing hypocalcemia. This novel route offers a promising alternative for managing parturient hypocalcemia in cattle.
Area of Science:
- Veterinary Medicine
- Endocrinology
- Animal Nutrition
Background:
- Parturient hypocalcemia is a significant metabolic disorder in dairy cows.
- Exogenous 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) is used to prevent hypocalcemia, typically administered via oral, intravenous, or intramuscular routes.
- Limited research exists on the intravaginal administration of 1,25(OH)2D3 in cattle.
Purpose of the Study:
- To investigate the efficacy and absorption of intravaginally administered 1,25(OH)2D3 in dairy heifers.
- To assess the impact of intravaginal 1,25(OH)2D3 on blood chemistry, including mineral levels and 1,25(OH)2D3 concentrations.
Main Methods:
- Five dairy heifers received a single intravaginal dose of 1 microg of 1,25(OH)2D3/kg body weight.
- One control heifer received the ethanol carrier alone.
- Blood samples were collected at various time points to measure plasma 1,25(OH)2D3, calcium, inorganic phosphate, and magnesium levels.
Main Results:
- Plasma 1,25(OH)2D3 levels increased significantly within 2 hours, peaking at 6 hours post-administration.
- Plasma calcium levels increased, reaching a peak at 24 hours.
- Plasma inorganic phosphate levels increased and plateaued, while plasma magnesium levels decreased and plateaued.
Conclusions:
- The study provides evidence for the successful absorption of exogenous 1,25(OH)2D3 through the bovine vaginal wall.
- Intravaginal administration of 1,25(OH)2D3 is a viable route for increasing circulating 1,25(OH)2D3 levels.
- This method shows potential utility for the prophylaxis of hypocalcemia in dairy cows.
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