Related Experiment Video
Updated: Aug 22, 2026

Ultrasonography-Guided Nasogastric Tube Placement and Verification: A Standardized Nursing Protocol
Published on: April 7, 2026
Methods to test feeding tube placement in children
1Saint Louis University School of Nursing, Saint Louis, MO, USA. westhusn@slu.edu
Insights
Testing feeding tube aspirates for pH and enzymes is a reliable method to confirm correct placement in acutely ill children. This simple bedside test, similar to adult protocols, ensures patient safety and accurate delivery of nutrition.
Area of Science:
- Pediatric critical care medicine
- Gastroenterology
- Clinical diagnostics
Background:
- Accurate placement of feeding tubes is crucial for effective nutritional support and preventing complications in acutely ill children.
- Current methods for confirming tube placement can be invasive or time-consuming.
- The effectiveness of using aspirate characteristics (pH, enzyme content) for tube placement confirmation in children requires further validation.
Purpose of the Study:
- To replicate findings from adult studies by examining the efficacy of feeding-tube aspirate characteristics (pH and enzyme content) as predictors of feeding tube location in pediatric patients.
- To determine if established methods for testing aspirates are adaptable and reliable for use in children.
Main Methods:
- A convenience sample of 56 children with nasogastric, orogastric, or nasointestinal tubes was studied.
- Aspirates (approx. 2.5 ml) were collected within 30 minutes of radiographic confirmation of tube placement.
- Aspirates were analyzed for pH and enzyme content, with results compared against radiographic findings.
Main Results:
- Gastric aspirates consistently showed lower pH and higher pepsin levels compared to intestinal aspirates.
- Trypsin levels were significantly higher in intestinal aspirates (mean 70 µg/ml) than gastric aspirates (mean 10 µg/ml).
- Visual inspection of aspirate color also differed between gastric and intestinal sites, with high predictive values (>90%) for all tested characteristics.
Conclusions:
- Methods for testing feeding tube aspirates, including pH and enzyme assays, are adaptable from adult to pediatric populations.
- Simple bedside tests, such as using pH test strips on feeding aspirates, can be reliably performed by nurses to confirm tube placement.
- The study provides evidence that indicators effective for confirming tube location in adults are also effective in children, enhancing patient safety and care.
Purpose:
This replication study examined the extent to which feeding-tube aspirates (pH and enzyme content) are effective predictors of feeding tube location in acutely ill children.
Study Design And Methods:
A convenience sample of 56 children was obtained at a metropolitan children's hospital. Approximately 2.5 ml of fluid were withdrawn from children's nasogastric, orogastric, or nasointestinal tubes within 30 minutes of radiographic examination. Fluid was tested for pH and enzymes, and results were compared with radiographic results of tube location.
Results:
Mean gastric pH was significantly lower than mean intestinal pH. Mean fasting gastric pepsin level was significantly higher than mean fasting intestinal pepsin level. Usual intestinal constituent, trypsin, was significantly higher in the small bowel than in the stomach. Mean fasting trypsin level was 70 microg/ml in the intestine, and only 10 microg/ml in the gastric site. Predictive positive value was >90% for all tests; predictive negative value was < 65%. Colors of the gastric aspirates were distinctly different from those observed in intestinal aspirates.
Clinical Implications:
Methods of aspirating fluid from feeding tubes and testing visually or assaying for aspirate characteristics were found to be adaptable from adults to children. The clinical implications of being able to use a test as simple as pH of feeding aspirates means that nurses can easily test these aspirates in any clinical setting using reliable pH test strips, similar to what was done in this study. These data add evidence that suggests indicators of tube location effective in adults are also effective in children.
Related Concept Videos
Enteral Nutrition I: Orogastric and Nasogastric Feeding
Orogastric (OG) and nasogastric (NG) feeding are two standard methods used for enteral nutrition. Enteral nutrition is often preferred over...
Enteral Nutrition II: Nasointestinal and Gastrostomy Feeding
Nasointestinal Feeding
Nasointestinal feeding involves placing a tube through...
Endotracheal Intubation I: Procedure
The ET tube comprises various components, including a standard adaptor to attach a bag-valve-mask (BVM) or ventilator, a cuff, a pilot balloon, and radiopaque markings along its length to measure the insertion distance. The tube sizes...
Parentral Nutrition: Centeral and Peripheral Parental Nutrition
PN can be administered through two primary routes:
1. Central Parenteral Nutrition (CPN):
CPN involves delivering a high concentration of nutrients through a large vein. This is typically achieved using a Peripherally Inserted Central Catheter (PICC) or,...
Endoscopic Procedures I: Esophagogastroduodenoscopy
During an EGD, the endoscope can be used to:
Tracheostomy: Procedure and Tubes
Tracheostomy tubes can be made of semiflexible plastic (polyurethane or silicone), rigid plastic, or metal, and they come in...