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Acetylcholine esterase protects LDL against oxidation.
Bianca Fuhrman1, Ayelet Partoush, Michael Aviram
1The Lipid Research Laboratory, Technion Faculty of Medicine, The Rappaport Family Institute for Research in the Medical Sciences and Rambam Medical Center, Haifa, Israel.
Biochemical and Biophysical Research Communications
|September 1, 2004
Summary
Acetylcholine esterase (AChE) protects low-density lipoprotein (LDL) against oxidation by breaking down lipid peroxides. This finding suggests AChE may help prevent atherosclerosis development.
Area of Science:
- Biochemistry
- Cardiovascular Science
- Molecular Biology
Background:
- Acetylcholine esterase (AChE) and paraoxonase 1 (PON1) are serum esterases linked to myocardial infarction.
- Both genes are located on chromosome 7q21-22.
- PON1 is known to protect against cardiovascular disease by metabolizing oxidized lipids.
Purpose of the Study:
- To investigate the potential of AChE to protect low-density lipoprotein (LDL) against oxidation.
- To compare the antioxidant properties of AChE with those of PON1.
Main Methods:
- LDL was preincubated with varying concentrations of AChE.
- Copper ion-induced LDL oxidation was monitored.
- Formation of lipid peroxides and TBARS was quantified.
- AChE's hydrolysis of lipid peroxides was assessed.
Main Results:
- AChE preincubation inhibited LDL oxidation in a concentration-dependent manner.
- AChE significantly reduced lipid peroxide and TBARS formation by up to 45%.
- AChE-mediated hydrolysis of lipid peroxides was identified as the mechanism of inhibition.
Conclusions:
- AChE, similar to PON1, possesses the ability to hydrolyze lipid peroxides.
- AChE may prevent the accumulation of oxidized LDL.
- AChE may play a role in attenuating atherosclerosis development.