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OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
Published on: June 20, 2010
Isolation of diferulic bridges ester-linked to arabinan in sugar beet cell walls
Sébastien Levigne1, Marie-Christine Ralet, Bernard Quéméner
1Unité de Recherche sur les Polysaccharides, leurs Organisations et Interactions, INRA, rue de la Géraudière, B.P. 71627, F-44316 Nantes Cedex 3, France.
Abstract:
After degradation of sugar beet cell walls with Driselase and fractionation of the solubilised products by hydrophobic interaction chromatography, a dehydrodiferuloylated oligoarabinan was isolated. Its structure was assigned to two dimers of (1-->5)-linked arabinose units esterified by a central 8-O-4' ferulic dimer. These results provide the first direct evidence that pectic arabinans in sugar beet cell walls may be covalently cross-linked through dehydrodiferulates.
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